Cysteine protease RD21A regulated by E3 ligase SINAT4 is required for drought-induced resistance to Pseudomonas syringae in Arabidopsis.
Identifieur interne : 000191 ( Main/Exploration ); précédent : 000190; suivant : 000192Cysteine protease RD21A regulated by E3 ligase SINAT4 is required for drought-induced resistance to Pseudomonas syringae in Arabidopsis.
Auteurs : Yi Liu [États-Unis, République populaire de Chine] ; Kunru Wang [États-Unis] ; Qiang Cheng [États-Unis] ; Danyu Kong [États-Unis] ; Xunzhong Zhang [États-Unis] ; Zhibo Wang [États-Unis] ; Qian Wang [République populaire de Chine] ; Qi Xie [République populaire de Chine] ; Jijun Yan [République populaire de Chine] ; Jinfang Chu [République populaire de Chine] ; Hong-Qing Ling [République populaire de Chine] ; Qi Li [États-Unis] ; Jiamin Miao [États-Unis, République populaire de Chine] ; Bingyu Zhao [États-Unis]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
Plants can be simultaneously exposed to multiple stresses. The interplay of abiotic and biotic stresses may result in synergistic or antagonistic effects on plant development and health. Temporary drought stress can stimulate plant immunity; however, the molecular mechanism of drought-induced immunity is largely unknown. In this study, we demonstrate that cysteine protease RD21A is required for drought-induced immunity. Temporarily drought-treated wild-type Arabidopsis plants became more sensitive to the bacterial pathogen-associated molecular pattern flg22, triggering stomatal closure, which resulted in increased resistance to Pseudomonas syringae pv. tomato DC3000 (Pst-DC3000). Knocking out rd21a inhibited flg22-triggered stomatal closure and compromised the drought-induced immunity. Ubiquitin E3 ligase SINAT4 interacted with RD21A and promoted its degradation in vivo. The overexpression of SINAT4 also consistently compromised the drought-induced immunity to Pst-DC3000. A bacterial type III effector, AvrRxo1, interacted with both SINAT4 and RD21A, enhancing SINAT4 activity and promoting the degradation of RD21A in vivo. Therefore, RD21A could be a positive regulator of drought-induced immunity, which could be targeted by pathogen virulence effectors during pathogenesis.
DOI: 10.1093/jxb/eraa255
PubMed: 32453812
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Jiangxi Province and Chinese Academy of Sciences, Jiujiang, Jiangxi</wicri:regionArea><wicri:noRegion>Jiangxi</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Kunru" sort="Wang, Kunru" uniqKey="Wang K" first="Kunru" last="Wang">Kunru Wang</name><affiliation wicri:level="2"><nlm:affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</nlm:affiliation><country 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USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Wang, Qian" sort="Wang, Qian" uniqKey="Wang Q" first="Qian" last="Wang">Qian Wang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xie, Qi" sort="Xie, Qi" uniqKey="Xie Q" first="Qi" last="Xie">Qi Xie</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Jijun" sort="Yan, Jijun" uniqKey="Yan J" first="Jijun" last="Yan">Jijun Yan</name><affiliation wicri:level="3"><nlm:affiliation>National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chu, Jinfang" sort="Chu, Jinfang" uniqKey="Chu J" first="Jinfang" last="Chu">Jinfang Chu</name><affiliation wicri:level="3"><nlm:affiliation>National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ling, Hong Qing" sort="Ling, Hong Qing" uniqKey="Ling H" first="Hong-Qing" last="Ling">Hong-Qing Ling</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Qi" sort="Li, Qi" uniqKey="Li Q" first="Qi" last="Li">Qi Li</name><affiliation wicri:level="2"><nlm:affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author><author><name sortKey="Miao, Jiamin" sort="Miao, Jiamin" uniqKey="Miao J" first="Jiamin" last="Miao">Jiamin Miao</name><affiliation wicri:level="2"><nlm:affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Grassland, Gansu Agricultural University, Lanzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Grassland, Gansu Agricultural University, Lanzhou</wicri:regionArea><wicri:noRegion>Lanzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Bingyu" sort="Zhao, Bingyu" uniqKey="Zhao B" first="Bingyu" last="Zhao">Bingyu Zhao</name><affiliation wicri:level="2"><nlm:affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA</wicri:regionArea><placeName><region type="state">Virginie</region></placeName></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants can be simultaneously exposed to multiple stresses. The interplay of abiotic and biotic stresses may result in synergistic or antagonistic effects on plant development and health. Temporary drought stress can stimulate plant immunity; however, the molecular mechanism of drought-induced immunity is largely unknown. In this study, we demonstrate that cysteine protease RD21A is required for drought-induced immunity. Temporarily drought-treated wild-type Arabidopsis plants became more sensitive to the bacterial pathogen-associated molecular pattern flg22, triggering stomatal closure, which resulted in increased resistance to Pseudomonas syringae pv. tomato DC3000 (Pst-DC3000). Knocking out rd21a inhibited flg22-triggered stomatal closure and compromised the drought-induced immunity. Ubiquitin E3 ligase SINAT4 interacted with RD21A and promoted its degradation in vivo. The overexpression of SINAT4 also consistently compromised the drought-induced immunity to Pst-DC3000. A bacterial type III effector, AvrRxo1, interacted with both SINAT4 and RD21A, enhancing SINAT4 activity and promoting the degradation of RD21A in vivo. Therefore, RD21A could be a positive regulator of drought-induced immunity, which could be targeted by pathogen virulence effectors during pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32453812</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><Issue>18</Issue><PubDate><Year>2020</Year><Month>Sep</Month><Day>19</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Cysteine protease RD21A regulated by E3 ligase SINAT4 is required for drought-induced resistance to Pseudomonas syringae in Arabidopsis.</ArticleTitle><Pagination><MedlinePgn>5562-5576</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/eraa255</ELocationID><Abstract><AbstractText>Plants can be simultaneously exposed to multiple stresses. The interplay of abiotic and biotic stresses may result in synergistic or antagonistic effects on plant development and health. Temporary drought stress can stimulate plant immunity; however, the molecular mechanism of drought-induced immunity is largely unknown. In this study, we demonstrate that cysteine protease RD21A is required for drought-induced immunity. Temporarily drought-treated wild-type Arabidopsis plants became more sensitive to the bacterial pathogen-associated molecular pattern flg22, triggering stomatal closure, which resulted in increased resistance to Pseudomonas syringae pv. tomato DC3000 (Pst-DC3000). Knocking out rd21a inhibited flg22-triggered stomatal closure and compromised the drought-induced immunity. Ubiquitin E3 ligase SINAT4 interacted with RD21A and promoted its degradation in vivo. The overexpression of SINAT4 also consistently compromised the drought-induced immunity to Pst-DC3000. A bacterial type III effector, AvrRxo1, interacted with both SINAT4 and RD21A, enhancing SINAT4 activity and promoting the degradation of RD21A in vivo. Therefore, RD21A could be a positive regulator of drought-induced immunity, which could be targeted by pathogen virulence effectors during pathogenesis.</AbstractText><CopyrightInformation>Published by Oxford University Press on behalf of the Society for Experimental Biology 2020.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Lushan Botanical Garden Jiangxi Province and Chinese Academy of Sciences, Jiujiang, Jiangxi, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Kunru</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kong</LastName><ForeName>Danyu</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xunzhong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhibo</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Jijun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chu</LastName><ForeName>Jinfang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ling</LastName><ForeName>Hong-Qing</ForeName><Initials>HQ</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Jiamin</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Grassland, Gansu Agricultural University, Lanzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Bingyu</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cysteine protease</Keyword><Keyword MajorTopicYN="N">RD21A</Keyword><Keyword MajorTopicYN="N">SINAT4</Keyword><Keyword MajorTopicYN="N">drought treatment</Keyword><Keyword MajorTopicYN="N">protein interactions</Keyword><Keyword MajorTopicYN="N">stomatal immunity</Keyword><Keyword MajorTopicYN="N">ubiquitin E3 ligase</Keyword><Keyword MajorTopicYN="N">virulence effector</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32453812</ArticleId><ArticleId IdType="pii">5846193</ArticleId><ArticleId IdType="doi">10.1093/jxb/eraa255</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Virginie</li></region><settlement><li>Pékin</li></settlement></list><tree><country name="États-Unis"><region name="Virginie"><name sortKey="Liu, Yi" sort="Liu, Yi" uniqKey="Liu Y" first="Yi" last="Liu">Yi Liu</name></region><name sortKey="Cheng, Qiang" sort="Cheng, Qiang" uniqKey="Cheng Q" first="Qiang" last="Cheng">Qiang Cheng</name><name sortKey="Kong, Danyu" sort="Kong, Danyu" uniqKey="Kong D" first="Danyu" last="Kong">Danyu Kong</name><name sortKey="Li, Qi" sort="Li, Qi" uniqKey="Li Q" first="Qi" last="Li">Qi Li</name><name sortKey="Miao, Jiamin" sort="Miao, Jiamin" uniqKey="Miao J" first="Jiamin" last="Miao">Jiamin Miao</name><name sortKey="Wang, Kunru" sort="Wang, Kunru" uniqKey="Wang K" first="Kunru" last="Wang">Kunru Wang</name><name sortKey="Wang, Zhibo" sort="Wang, Zhibo" uniqKey="Wang Z" first="Zhibo" last="Wang">Zhibo Wang</name><name sortKey="Zhang, Xunzhong" sort="Zhang, Xunzhong" uniqKey="Zhang X" first="Xunzhong" last="Zhang">Xunzhong Zhang</name><name sortKey="Zhao, Bingyu" sort="Zhao, Bingyu" uniqKey="Zhao B" first="Bingyu" last="Zhao">Bingyu Zhao</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Yi" sort="Liu, Yi" uniqKey="Liu Y" first="Yi" last="Liu">Yi Liu</name></noRegion><name sortKey="Chu, Jinfang" sort="Chu, Jinfang" uniqKey="Chu J" first="Jinfang" last="Chu">Jinfang Chu</name><name sortKey="Ling, Hong Qing" sort="Ling, Hong Qing" uniqKey="Ling H" first="Hong-Qing" last="Ling">Hong-Qing Ling</name><name sortKey="Liu, Yi" sort="Liu, Yi" uniqKey="Liu Y" first="Yi" last="Liu">Yi Liu</name><name sortKey="Miao, Jiamin" sort="Miao, Jiamin" uniqKey="Miao J" first="Jiamin" last="Miao">Jiamin Miao</name><name sortKey="Wang, Qian" sort="Wang, Qian" uniqKey="Wang Q" first="Qian" last="Wang">Qian Wang</name><name sortKey="Xie, Qi" sort="Xie, Qi" uniqKey="Xie Q" first="Qi" last="Xie">Qi Xie</name><name sortKey="Yan, Jijun" sort="Yan, Jijun" uniqKey="Yan J" first="Jijun" last="Yan">Jijun Yan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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